Entry: Q57532 Genes: gyrA ML0006 Length: 1273 aa

DNA gyrase subunit A

GYRA_MYCLE • Mycobacterium leprae (strain TN)
Navigate Overview Model Scores RCSB 1D Mol* Pockets B-Cell Epitopes Function and interactions Ontology and localisation Sequence features and PTMs Evidence and cross-references

Summary

Download FASTA UniProt: Q57532 ML Locus: ML0006
Entry Q57532
Reviewed reviewed
Entry Name GYRA_MYCLE
Gene Names gyrA ML0006
ML locus tag ML0006
Organism Mycobacterium leprae (strain TN)
Length 1273 aa
Mass 141218 Da
Annotation 5.0
Annotation Summary

Evidence and cross-references

PubMed ID 8622949; 11234002; 8540734; 17325221
DOI ID 10.1073/pnas.93.8.3410; 10.1038/35059006; 10.1128/aac.39.9.2145; 10.1128/aac.01282-06
STRING 272631.gene:17573815;
KEGG mle:ML0006;
eggNOG COG0188;COG1372;
InterPro IPR006691;IPR035516;IPR003586;IPR003587;IPR036844;IPR027434;IPR006142;IPR030934;IPR004042;IPR006141;IPR004860;IPR013760;IPR013758;IPR013757;IPR002205;IPR053555;IPR050220;

Oligomer Models

Best Boltz2 oligomer and local AlphaFold‑3 homooligomer structure models matched to ML0006, with the same model switching, Mol*, Nightingale, pLDDT, PAE, and download functionality as monomers.
Count: 1
Assembly Method Template / Source Overall Rank Download
hetero_4mer Boltz2 — — Download Model

Ligands

Curated ligands imported from the selected oligomer template/YAML rows. The displayed ligand code is the original template CCD code; predicted-model placeholders such as LIG1/LIG2 are not shown. Click a ligand code to focus the ligand in Mol*.
Ligands: 0
Ligand Code Ligand Name PubChem ID
No curated ligand rows were found for this oligomer in the database.

Monomer Models

Monomer structure models for ML0006, retaining all existing model selection, Mol*, Nightingale, pLDDT, PAE, and download functionality.
Count: 4
Assembly Method Model Overall Rank Download
Monomer Boltz2 0 0.808386 Download Model
Monomer AlphaFold‑3 1 0.674110 Download Model
Monomer Boltz 0 0.814921 Download Model
Monomer CHAI 1 0.153089 Download Model

Sequence & Feature Viewer

Residue-level feature tracks for the loaded model — pLDDT confidence colouring, mapped annotations, and interactive PAE selection ranges. Drag a region on the heatmap to highlight it here and in Mol*.
RCSB 1D
Sequence viewer will initialise once a structure model is loaded.
Mol* — ML0006

Mol* Structure Viewer

Local mmCIF model display for ML0006 with Mol* controls hidden.
Default: hetero_4mer • Boltz2
Preparing hetero_4mer • Boltz2 model 0 for ML0006…
Residue focus: none
Heterooligomer ML IDs / chain selection

PAE Map Panel

Predicted aligned error heatmap for the currently loaded model, when pairwise confidence data are available in the local mmCIF.
PAE • Waiting for Model
PAE Heatmap
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Scored residue
Aligned residue
Hover over the heatmap to inspect residue-pair PAE values.
Click a cell or drag across the heatmap to select residue blocks and mirror them in the sequence viewer.

B-cell Epitope Propensity

Per-residue conformational B-cell epitope predictions from DiscoTope-3.0.
Epitope · Loading
Low
Epitope
Residue number Residue type DiscoTope-3.0 score Focus
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Click a residue to focus it in Mol*.
Annotation Summary

Function and interactions

EC number 5.6.2.2
Catalytic activity CATALYTIC ACTIVITY: Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-Rule:MF_01897};
Active site ACT_SITE 130; /note="O-(5'-phospho-DNA)-tyrosine intermediate"; /evidence="ECO:0000255|HAMAP-Rule:MF_01897"
Subunit structure SUBUNIT: Heterotetramer, composed of two GyrA and two GyrB chains (PubMed:17325221). In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with the DNA, while GyrB binds cofactors catalyzes ATP hydrolysis. {ECO:0000255|HAMAP-Rule:MF_01897, ECO:0000269|PubMed:17325221}.
Annotation Summary

Ontology and localisation

Gene Ontology (biological process) DNA topological change [GO:0006265]; intein-mediated protein splicing [GO:0016539]
Gene Ontology (cellular component) cytoplasm [GO:0005737]; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex [GO:0009330]
Gene Ontology (GO) cytoplasm [GO:0005737]; DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex [GO:0009330]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA negative supercoiling activity [GO:0034335]; endonuclease activity [GO:0004519]; DNA topological change [GO:0006265]; intein-mediated protein splicing [GO:0016539]
Gene Ontology (molecular function) ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA negative supercoiling activity [GO:0034335]; endonuclease activity [GO:0004519]
Gene Ontology IDs GO:0003677; GO:0004519; GO:0005524; GO:0005737; GO:0006265; GO:0009330; GO:0016539; GO:0034335
Subcellular location [CC] SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01897}.
Protein families Type II topoisomerase GyrA/ParC subunit family
Sequence similarities SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit family. {ECO:0000255|HAMAP-Rule:MF_01897}.
Annotation Summary

Sequence features and PTMs

Chain CHAIN 1..130; /note="DNA gyrase subunit A, 1st part"; /evidence="ECO:0000250"; /id="PRO_0000034807"; CHAIN 131..550; /note="Mle GyrA intein"; /evidence="ECO:0000250"; /id="PRO_0000034808"; CHAIN 551..1273; /note="DNA gyrase subunit A, 2nd part"; /evidence="ECO:0000250"; /id="PRO_0000034809"
Post-translational modification PTM: This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. {ECO:0000305}.
Motif MOTIF 958..964; /note="GyrA-box"; /evidence="ECO:0000255|HAMAP-Rule:MF_01897"
Domain [FT] DOMAIN 42..931; /note="Topo IIA-type catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU01384"; DOMAIN 256..396; /note="DOD-type homing endonuclease"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"